De Novo Sequencing and Resurrection of a Human Astrovirus-Neutralizing Antibody.

TitleDe Novo Sequencing and Resurrection of a Human Astrovirus-Neutralizing Antibody.
Publication TypeJournal Article
Year of Publication2016
AuthorsBogdanoff, Walter A., Morgenstern David, Bern Marshall, Ueberheide Beatrix M., Sanchez-Fauquier Alicia, and DuBois Rebecca M.
JournalACS Infect Dis
Date Published2016 May 13

Monoclonal antibody (mAb) therapeutics targeting cancer, autoimmune diseases, inflammatory diseases, and infectious diseases are growing exponentially. Although numerous panels of mAbs targeting infectious disease agents have been developed, their progression into clinically useful mAbs is often hindered by the lack of sequence information and/or loss of hybridoma cells that produce them. Here we combine the power of crystallography and mass spectrometry to determine the amino acid sequence and glycosylation modification of the Fab fragment of a potent human astrovirus-neutralizing mAb. We used this information to engineer a recombinant antibody single-chain variable fragment that has the same specificity as the parent monoclonal antibody to bind to the astrovirus capsid protein. This antibody can now potentially be developed as a therapeutic and diagnostic agent.

Alternate JournalACS Infect Dis
PubMed ID27213181
PubMed Central IDPMC4869151